Bitcoin Cryonics Fund

Agreed, emphatically, that we need more funding to go for empirical validation of cryonics premises and procedures. However, Aschwin de Wolf has pointed out one reason it is difficult to compare vitrified brains to fixed-embedded, namely dehydration. So I'm somewhat suspicious of the usefulness of the prize at this point in time.

A specific concern for Brain Preservation Technology Prize competitors using cryopreservation is that cryopreserved brains are currently very dehydrated. Due to this dehydration, which typically persists even after cryoprotectant removal, it is not yet clear that cryopreserved brains can be effectively evaluated by the Prize organizers. To be specific, the criterion for success is preservation of the connectome, which requires two things: preservation of synapses and preservation of enough information to infer the pattern of connections between them. Neural cryobiology researchers believe that they can achieve good ultrastructural preservation of the brain but dehydration compactifies the neuropil, reduces space between structures, and makes the tissue so dark in the electron microscope that it is hard to actually observe the synapses. So if a quick scanning method doesn’t discern all synapses that are actually there, it will fail. There are techniques for doing electron microscopy at cryogenic temperatures in the vitrified state, but these depend on the tissue being sliced before vitrification. Making slices out of a whole vitrified brain while vitrified is a tough problem. It is easier to make thin slices out of a whole brain that’s been turned into solid plastic because the resin used is designed for being cut into thin slices for microscopy. So plastination has a natural advantage in this competition — in terms of processing for the tests rather than in actual results.

A non-dehydrated brain is not only more likely to survive by information-theoretic criteria, and to e.g. benefit from ice-blockers, it is easier to scan for evidence of survival. So a high priority should be to achieve reduction in dehydration. An earlier discussion with Mike Darwin had me thinking it was a problem with myelination, but currently I am given to understand (per Aschwin de Wolf and Greg Fahy) that it is primarily a matter of the blood-brain barrier. Aschwin also mentioned that there should be evidence in the literature of what ideas to try next, since this is a problem in conventional drug delivery. Yuri Pichigu has developed an approach to opening the BBB, however 21CM found that it did not increase viability in rabbit brain slices. I'm not sure whether that failure is limited to viability or also extends to the morphological characteristics we are interested in here.

Anyway, dehydration and the blood-brain barrier is high in the search order for the next thing to solve. Without it, vitrification is a bit of a shot in the dark. We can speculate that dehydration is okay as long as it is relatively uniform, but it may not be (and of course isn't, in more poorly perfused patients).

OpenWorm could use some money too: http://www.openworm.org/donate.html

a simple experiment like vitrifying & reviving some C. elegans or something to see if they retain learned memories like maze-running

And it's done! "Persistence of Long-Term Memory in Vitrified and Revived C. elegans", Vita-More & Barranco 2015:

Can memory be retained after cryopreservation? Our research has attempted to answer this long-standing question by using the nematode worm Caenorhabditis elegans (C. elegans), a well-known model organism for biological research that has generated revolutionary findings but has not been tested for memory retention after cryopreservation. Our study’s goal was to test C. elegans’ memory recall after vitrification and reviving. Using a method of sensory imprinting in the young C. elegans we established that learning acquired through olfactory cues shapes the animal’s behavior and the learning is retained at the adult stage after vitrification. Our research method included olfactory imprinting with the chemical benzaldehyde (C6H5CHO) for phase-sense olfactory imprinting at the L1 stage, the fast cooling SafeSpeed method for vitrification at the L2 stage, reviving, and a chemotaxis assay for testing memory retention of learning at the adult stage. Our results in testing memory retention after cryopreservation show that the mechanisms that regulate the odorant imprinting (a form of long-term memory) in C. elegans have not been modified by the process of vitrification or by slow freezing.

Following up on this: I contacted someone at BPF, and they say they have some initial images they will be posting soon.

They both fall under the category of a highly underestimated long bets in my mind. Bitcoin's recent rise in value vindicates my earlier speculative feelings and makes me wish I had listened to them more consistently (not selling out my personal stock, e.g.). So I should be more confident associating it with cryonics than I was before.

If you think of them as simple independent variables, success for both cryonics and bitcoin would appear a less probable conjunction than success for either cryonics or bitcoin. However popularity can drive technology and vice versa in both areas. So given that both are early-adopter material at present anyway, it is plausible that they would complement / benefit from association with each other overall.

Another point is that cryonics encourages long-term thinking about end of life / post-death long term trusts for patient care. If we assume the thing that makes it harder to win in bitcoin is the urge to spend too soon, this gives cryonicists a competitive advantage.

I'd advise caution to everyone if they attempt to combine paypal and bitcoin -- see here

As far as I can tell, the recent rise in Bitcoin prices is overwhelmingly due to speculation, and is probably a repeat of the 2011 bubble on a larger scale. If you google "bitcoin bubble", there's lots of info on this.

I'm not a quant, but if you're holding bitcoins, I'd recommend selling off half of them and then rebalancing (keeping the value of your MtGox account 50% bitcoins and 50% dollars) going forward, which basically allows you to make money off of volatility in Bitcoin (while also having the potentially useful side effect of stabilizing its price and making it more viable as an alternative currency). (You can read about the theory behind rebalancing here.)

Most mined coins never hit the open market. Many miners are only recouping equipment and electricity costs and holding the rest.

The market is still very thin, though thickening a bit in the last couple weeks.

Does the code automatically put an article to the top when it's edited after saving? Might want to fix that.

(I'd expect something like: set its date in the timeline at the moment it goes from "draft" to "published". Not sure what would be a senslble way to handle putting it back from "published" to "draft"; possibly just ignore that case, and allow the social process to deal appropriately per case with posts that have been bumped. I'm assuming lsparrish was just intending to add a note to the top of the article, not specifically to bump it to the top.)

The post's submission date now say today (April 14th). Maybe the author has been editing the post that increased the post's rank.